Nicolas Dauguet
CYTF Core Facility Manager
Flow Cytometry, Cell Sorting & Single-Cell Analysis - CYTF
Nicolas Dauguet
Institut de Duve
Avenue Hippocrate, 74 - B1.74.04
1200 Bruxelles
CYTF core facility is managed by Dr Nicolas Dauguet. Flow cytometry technology allows simultaneous multiparametric analysis of thousands of cells per second, enabling trained users to rapidly analyze complex cell populations based on phenotypic and functional features.
High-speed assisted cell sorting services provide researchers with physical separation of identified cell populations, for any down-stream characterizations.
The main mission of the platform is to provide a high quality service and assist researchers with their flow cytometry experiments. Another key point is to provide a high-level of education to the researchers so that they achieve autonomy with their research projects. It means autonomy with the use of the equipments but also with the design of their experiments and data analysis.
Equipment
Flow cytometer analysers
- BD FACSVerse (3L ; FSC/SSC + 8 Fluorescent parameters)
- BD LSR Fortessa (4L-YG ; FSC/SSC + 16 Fluorescent parameters)
- Cytek Aurora (4L-YG ; FSC/SSC-B/SSC-V + full spectrum)
Cell sorters
- BD FACS ARIA III (4L-YG ; FSC/SSC + 16 Fluorescent parameters)
- Sony MA900 (4L ; FSC/SSC + 12 fluorescent parameters)
- Cytek Aurora CS (4L-YG ; FSC/SSC-B/SSC-V + full spectrum)
scRNAseq
- 10x Genomics : chromium Platform
- MANTIS® - Microfluidic Liquid Dispenser (Formulatrix) for Smart-seq2
Offered service
Flow Cytometry training course on-demand : theory, hands-on, data analysis
Flowjo and SpectroFlo analysis softwares
BD FACS ARIA III : assisted cell sorting
Sony MA900 : autonomous access
Cytek Aurora CS : assisted cell sorting, available soon
scRNAseq: contact Pr. Laure Dumoutier
ImageXpress-Pico
Major achievements
- “Advanced Technology Equipment” grant from the Fondation contre le Cancer. Acquisition of the full spectrum cell sorter Cytek Aurora CS. 2022
- “Grand équipement” grant from the FNRS. Acquisition of the full spectrum analyser Cytek Aurora. 2021
Team
Academic supervisor
Research assistant
A Mouse Model of Cholangiocarcinoma Uncovers a Role for Tensin-4 in Tumor Progression.
Di-Luoffo M, Pirenne S, Saandi T, Loriot A, Gérard C, Dauguet N, Manzano-Núñez F, Alves Souza Carvalhais N, Lamoline F, Cordi S, Konobrocka K, De Greef V, Komuta M, Halder G, Jacquemin P, Frédéric L, Lemaigre FP
Hepatology (2021) 74(3):1445-1460.
A targetable, noncanonical signal transducer and activator of transcription 3 activation induced by the Y-Less Region of IL-22 receptor orchestrates Imiquimod-induced psoriasis-like dermatitis in mice.
Michiels C, Puigdevall L, Cochez P, Achouri Y, Cheou P, Hendrickx E, Dauguet N, Blanchetot C, Dumoutier L
J Invest Dermatol (2021) 141(11):2668-2678.e6.
AhR modulates the IL-22-producing cell proliferation/recruitment in imiquimod-induced psoriasis mouse model.
Cochez PM, Michiels C, Hendrickx E, Van Belle AB, Lemaire MM, Dauguet N, Warnier G, de Heusch M, Togbe D, Ryffel B, Coulie PG, Renauld JC, Dumoutier L
Eur J Immunol (2016) 46(6):1449-59
Avenue Hippocrate 75 - 1200 Brussels - BELGIUM
VAT : BE0423 837 837
Tel : +32 2 764 75 50